Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354
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چکیده
منابع مشابه
Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354.
We have used random chemical mutagenesis and a simple genetic screen to generate and isolate a thermostable mutant of luciferase from the North American firefly (Photinus pyralis). A single G-to-A transition mutation, resulting in the substitution of a glutamate for a lysine residue at position 354 in the protein sequence, was shown to be responsible for this enhanced thermostability. Replaceme...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1996
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3190343